1 results about "Viral antigens" patented technology

The invention relates to the technical field of biomedical engineering and discloses a production method of fusion protein (F-Fc) of RSV (respiratory syncytial virus) antigen protein F and antibody constant region Fc, and application of the fusion protein as RSV subunit vaccine. The method includes: establishing a eukaryotic expression vector of the fusion protein containing RSV protein F and human IgG2Fc by molecular cloning, transfecting CHO (Chinese hamster ovary) cells, and expressing the fusion protein; performing protein A affinity chromatography to obtain high-purity fusion protein; performing fusion protein nasal dripping to immunize BABL/c mice to trigger specific mucosal immunity against the RSV, humoral immunity and 'Th1/Th2 balanced' cellular immunity partial to Th1 type, and thereby effectively inhibiting RSV infections.