Simultaneous solid phase extraction and detection method of a plurality of allergic aroma agents in toys

[0027] Example 1

[0028] Pretreatment and determination methods of 45 allergic fragrances in wooden toys:

[0029] (1) Extraction:

[0030] Cut the component to be tested into small pieces, accurately weigh 1.0235g of the sample in a ground bottle, accurate to 0.1mg, add 10mL of tetrahydrofuran, make sure that all the samples are immersed in the solvent, if not enough, you need to reduce the weight appropriately Or increase the extraction solvent. The mouth of the bottle was sealed with a ground stopper, and ultrasonically extracted at room temperature in an ultrasonic cleaner for 30 minutes, and the extract was centrifuged.

[0031] (2) Remove impurities

[0032] Activate the neutral alumina cartridge (1g / 3mL) with 3~5mL tetrahydrofuran, taking care not to let the tetrahydrofuran drain. Load the extract obtained by ultrasound, wash the sample container with 1~2mL of tetrahydrofuran, and rinse the small column, drain under low vacuum, collect the column loading solution and eluent, d

[0042] Example 2

[0043] Pretreatment and determination methods of 45 allergic fragrances in plush toys:

[0044] (1) Extraction:

[0045] Accurately weigh 1.0764g (accurate to 0.1mg) of red wool, add 10mL of tetrahydrofuran, and ensure that all samples are immersed in the solvent. If this is not possible, reduce the weighing amount or increase the extraction solvent. The mouth of the bottle was sealed with a ground stopper, and ultrasonically extracted at room temperature in an ultrasonic cleaner for 30 minutes, and the extract was centrifuged.

[0046] (2) Remove impurities

[0047] Activate the neutral alumina cartridge (1g / 3mL) with 3~5mL tetrahydrofuran, taking care not to let the tetrahydrofuran drain. Load the extract obtained by ultrasound, wash the sample container with 1~2mL of tetrahydrofuran, and rinse the small column, drain under low vacuum, collect the column loading solution and eluent, dilute the volume to 15mL with tetrahydrofuran, and organic the solution To be tes

[0054] Example 3

[0055] Pretreatment and determination methods of 45 allergic fragrances in plastic toys:

[0056] (1) Extraction:

[0057] Accurately weigh 1.0753g (accurate to 0.1mg) of purple plastic, add 10mL of tetrahydrofuran, and ensure that all samples are immersed in the solvent. If this is not possible, reduce the sample weight or increase the extraction solvent. The mouth of the bottle was sealed with a ground stopper, and ultrasonically extracted at room temperature in an ultrasonic cleaner for 30 minutes, and the extract was centrifuged.

[0058] (2) Remove impurities

[0059] Activate the neutral alumina cartridge (1g / 3mL) with 3~5mL tetrahydrofuran, taking care not to let the tetrahydrofuran drain. Load the extract obtained by ultrasound, wash the sample container with 1~2mL of tetrahydrofuran, and rinse the small column, drain under low vacuum, collect the column loading solution and eluent, dilute the volume to 15mL with tetrahydrofuran, and organic the solution To