Tissue culture propagation method for Polygala fallax Hemsl

A technique of tissue culture of chrysanthemums, applied in the field of plant tissue culture, which can solve problems such as resource shortage, limited wild resources, and difficulty in harvesting, and achieve the effect of accelerating the reproduction speed

Inactive Publication Date: 2012-08-22
地缘(厦门)生物科技有限公司
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Problems solved by technology

[0005] Therefore, in view of the above-mentioned problems, the present invention provides a method for tissue culture and propagation of chrysanthemum chrysalis, which can obtain a large amount of chrysanthemum tissue culture seedlings in a short period of time, effectively solving the problem of low output of chrysanthemum chrysalis and harvesting. Harvesting difficulties, limited wild resources lead to severe shortage of resources, etc., and at the same time lay the foundation for the large-scale production of high-quality seedlings and even the cultivation and utilization of resources

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[0017] The implementation of the present invention will be described in detail below with reference to specific embodiments, so as to fully understand how the present invention applies technical means to solve technical problems and achieve the realization process of technical effects and implement them accordingly.

[0018] The embodiment of the present invention is:

[0019] A method for tissue culture and propagation of Pseudomonas aeruginosa includes the following steps:

[0020] (1) Collection and disinfection of explants ： On a sunny day in April, take the young stems with axillary buds from the same year as the explants of P. sylvestris, soak them in detergent for 10 minutes, gently scrub the surface of the stems with a soft brush, rinse them with running water for 30 minutes, and place them in ultra On a clean bench, cut into stem segments with 1-2 internodes, soak them in KMnO4 with a mass concentration of 0.1% for 5 min, rinse twice with sterile water, and soak them in ethanol

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Abstract

The invention relates to the technical field of plant tissue culture and provides a tissue culture propagation method for Polygala fallax Hemsl. By utilizing the method, a large amount of tissue culture seedlings of Polygala fallax Hemsl can be obtained in a short term, which effectively solves the problems of low yield, difficulty in harvest and severe resource shortage caused by limited wild resources and the like of the Polygala fallax Hemsl, and lays a foundation for scale production of high-quality nursery seedling of the Polygala fallax Hemsl and resource cultivation and utilization. The invention mainly comprises the following steps of: (1) collection and sterilization of an explant; (2) induction of adventitious bud; (3) enrichment culture of adventitious bud; (4) rooting culture of adventitious bud, and (5) hardening-seedling and transplantation.

Description

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Claims

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Application Information

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Owner 地缘(厦门)生物科技有限公司
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