Indoor auxiliary sweet potato drought tolerance identification method

A technology of auxiliary identification and drought resistance, applied in horticultural methods, botanical equipment and methods, horticulture, etc. Actionable effect

Inactive Publication Date: 2015-01-07
JIANGSU XUHUAI DISTRICT XUZHOU AGRI SCI INST +1
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

In order to promote the screening and identification of sweet potato drought-tolerant resources and other materials, predecessors have used shade to simulate drought treatment to identify sweet potato drought tolerance, but this requires a lot of manpower and material resources, and the workload is very large. Difficult to meet the initial screening and identification of a large number of materials
However, by studying the relationship between the changes in antioxidant physiological indicators and the drought tolerance of sweet potato under stress and contro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0012] Example 1,

[0013] 1. Test materials and methods

[0014] 1.1 Test materials: The materials selected for the test are 6 materials that have passed the Xinjiang drought tolerance identification base and have been identified as strong, medium and poor drought tolerance for two consecutive years. among them:

[0015] Strong drought tolerance: Wanzi 56, Guangshu 87;

[0016] Medium drought tolerance: Xushu 18, Xu 20-1;

[0017] Poor drought tolerance: Ningzishu No. 1 and Rizi 0602.

[0018] 1.2 Test method:

[0019] Configuration of 1 / 2MS solid medium: MS basic medium is halved of macroelement and trace element, add 30g / L of sucrose, 5g / L of Phytagel, adjust PH 5.7 before sterilization; after sterilization, divide into petri dishes with a diameter of 90mm , Cool for later use.

[0020] The configuration of hypertonic identification medium: First, according to W / V of 50g / L, 100g / L, 200g / L, 300g / L, under aseptic conditions, dissolve polyethylene glycol 8000 in 1 / 2MS liq

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Abstract

The invention relates to a sweet potato drought tolerance identification method, and in particular relates to an indoor auxiliary sweet potato drought tolerance identification method, belonging to the technical field of sweet potato drought tolerance identification. The method comprises the following steps: by selecting a test tube seedling stem section which is subjected to subculture for more than or just 50 days as an explant, cutting a single section, inoculating the explant into a high-permeation identification culture medium, performing inoculation treatment for 30 days, subsequently surveying the relative root growth number coefficients of different varieties, and judging the drought tolerance grade of the varieties according to the relative root growth number coefficients of different varieties, wherein the drought tolerance of the variety of which the relative root growth number coefficient is greater than or equal to 1.0 is high, the drought tolerance of the variety of which the relative root growth number coefficient is greater than 0.5 and less than 1.0 is medium, and the drought tolerance of the variety of which the relative root growth number coefficient is smaller than or equal to 0.5 is relatively poor. The method is simple to operate, is flexible and convenient and applicable to screening on a great number of materials, can relieve the workload of field drought tolerance identification and can improve the working efficiency.

Description

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Claims

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Application Information

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Owner JIANGSU XUHUAI DISTRICT XUZHOU AGRI SCI INST
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