A composition comprising mannoheptulose for use in the treatment or prevention of overweight and obesity

[0132] Example 1 - Preparation of Avocado Extract

[0133] Avocado extracts containing elevated levels of mannoheptulose were prepared according to the following optional methods and used in the compositions of the present examples.

[0134] Whole avocado fruit (approximately 900 kg) is provided. The fruit was split and pitted partially or entirely, providing about 225kg of pitted avocado halves. The raw avocado was charged into the grinder and, after some stirring, water (about 3000 kg) and CELLUBRIX (commercially available from Novozymes A / S) (about 1 liter). The mixture was stirred again while heating to about 66°C. After charging was complete, additional CELLUBRIX (about 1 L) was added and the entire mixture was stirred with CELLUBRIX at a controlled pH of about 5.5 for about 12 hours. The temperature is then increased to about 80°C and held for at least about 2 hours. The resulting digested plant mixture was then filtered at 80°C to provide the carbohydrate extract a

[0135] Example 2-The effect of mHep on mesenchymal stem cell (MSC) differentiation

[0136] Study 1 - This study describes an in vitro assay for testing the effect of mHep on the terminal differentiation of mesenchymal stem cells (MSCs) (ie adipose stem cells) into functional adipocytes. at 37°C and 5% CO 2 MSCs (C57BL / 6) (Gibco) were cultured in adipocyte differentiation medium (Invitrogen) in the presence of palmitate to induce preadipocytes to functional adipocytes MSC differentiation. MSC control plates were grown in basal medium without ADP (BP) as a negative control (ie reflecting undifferentiated MSCs).