Method for producing astaxanthin by using starch waste water

[0034] Example 1

[0035] A method utilizing starch waste water to produce astaxanthin, comprising the steps of:

[0036] Step 1) Pretreatment of starch wastewater: starch wastewater first enters the sedimentation tank for 24 hours, and then enters the acid-base adjustment tank to adjust the pH to 7.0;

[0037] Step 2) Preparation of mixed bacterial solution: Inoculate Bacillus amyloliquefaciens on LB solid medium for culture to obtain a single colony; pick a single colony and inoculate it on LB liquid medium for expansion culture, and obtain a concentration of 5×10 8 Bacillus amyloliquefaciens culture fluid of cfu / ml;

[0038] Inoculate Lactobacillus plantarum on MRS solid medium for culture to obtain a single colony, and then insert it into MRS liquid medium for expanded culture, and cultivate to a concentration of 5×10 8 Lactobacillus plantarum culture solution of cfu / ml;

[0039] Streak culture of Rhodopseudomonas palustris on the LB plate to obtain a single colony; pi

[0043] Example 2

[0044] A method utilizing starch waste water to produce astaxanthin, comprising the steps of:

[0045] Step 1) Pretreatment of starch wastewater: starch wastewater first enters the sedimentation tank for 12 hours, and then enters the acid-base adjustment tank to adjust the pH to 7.5;

[0046] Step 2) Preparation of mixed bacterial solution: Inoculate Bacillus amyloliquefaciens on LB solid medium for culture to obtain a single colony; pick a single colony and inoculate it on LB liquid medium for expansion culture, and obtain a concentration of 7×10 8 Bacillus amyloliquefaciens culture fluid of cfu / ml;

[0047] Inoculate Lactobacillus plantarum on MRS solid medium for culture to obtain a single colony, and then insert it into MRS liquid medium for expanded culture, and cultivate to a concentration of 3×10 8 Lactobacillus plantarum culture solution of cfu / ml;

[0048] Streak culture of Rhodopseudomonas palustris on LB plates to obtain a single colony; pick

[0052] Example 3

[0053] The treatment effect of composite bacterial liquid of the present invention to starch wastewater:

[0054] Taking the composite bacterial solution of Example 1 as an example, the control group 1 is two bacterial strains of Bacillus amyloliquefaciens and Lactobacillus plantarum, and the rest are the same as in Example 1; The control group 2 is Lactobacillus plantarum and Rhodopseudomonas palustris, and the rest are the same Embodiment 1; Control group 3 is Bacillus amyloliquefaciens and Rhodopseudomonas palustris, all the other are the same as embodiment 1. The specific results are shown in Table 1:

[0055] Table 1

[0056]

[0057] As shown in Table 1, after microbial treatment, the pollutants in the wastewater are greatly reduced, which is suitable for cultivating Haematococcus pluvialis.