Storage stable liquid formulation comprising a laccase

[0036] Example 5 describes the effect of increasing the pH value from 9 to 10 and is shown in Figure 4 in. Myceliophthora thermophila laccase appears to be more stable at pH 9.0, and after storage at 25°C and 40°C for 4 weeks, the activity still retains 93% and 84%. Mesh porous bacteria laccase preparation

[0037] In addition, as disclosed in WO 96 / 00290 (Novo Nordisk), when syringaldazine is used as a substrate, the optimal pH of the recombinant pore bacteria laccase is 5 to 5.5.

[0038] According to the present invention, the pH of the liquid preparation containing laccase and polyol derived from Porosus sieve is more alkaline than pH 5 to 5.5, preferably about pH 5.5 to 11, especially about pH 6 to 9.5.

[0039] As shown in Example 1, the laccase of the mesh porous bacteria laccase in a 60% PEG6000 liquid with a pH of 9.0, after 14 days at 40°C, the remaining activity is 100%, and after 4 weeks, the remaining activity is 65%. Polyol

[0040] In order to improve the storage stab

[0174] At pH 7.0, 8.0 and 9.0, the storage stability of liquid preparations containing high-purity sieve porous bacteria laccase (10LACU / g) and two different polyols were tested.

[0175] Preparation

[0176] It can be seen from Table 1 and Table 2 that in the presence of polyols, the storage stability is improved. Example 3 Storage stability of sieve porous bacteria laccase (4 weeks)

[0177] Preparation

[0178] It can be seen from Table 3 that after 4 weeks, significant residual activity was maintained at a higher pH value. Example 4 Storage stability of Myceliophthora thermophila laccase