The invention provides a preparation method of a 5-amido-1,2,4-selenadiazole derivative. The derivative comprises
amidine hydrochloride shown in a formula (I) and an isonitrile compound shown in a formula (II), and
selenium powder is subjected to a reaction on the condition of existence of an alkali compound to obtain the 5-amido-1,2,4-selenadiazole derivative. According to the preparation method, nontoxic
selenium powder is adopted as a
selenium source, no
metal participation is needed, and only organic alkali and
air atmosphere are needed; meanwhile, the adopted
amidine hydrochloride and isonitrile are wide in structure range,
aryl or
alkyl substrates can be subjected to a reaction smoothly, and the high substrate universality is achieved; operation is easy, only simple heating is needed, the reaction can be completed within 4-6 h, the reaction time is short, and meanwhile the reaction yield can reach 80-90%.