The invention discloses a wound protecting composition with bleeding-stopping and anti-bacterial functions and a preparation method of the wound protecting composition. The wound protecting composition comprises the following steps: (1) preparing an organic high molecular mixed solution; (2) adding one or two of chitosan and chitin into acetic acid, ethanol and water so as to prepare a bleeding-stopping and anti-bacterial material solution; (3) carrying out multi-spray-head electrostatic spinning so as to prepare a composite fiber material; and (4) drying the composite fiber material, removing residual solvents, and carrying out hot-press shaping, so as to prepare the wound protecting composition with the bleeding-stopping and anti-bacterial functions. The wound protecting composition disclosed by the invention has good bleeding-stopping and anti-bacterial effects, can be used for cleaning wounds and is good in biocompatibility; the water absorption rate is obviously increased, the bleeding-stopping time is greatly shortened, and the wounds are further protected.