The invention discloses a
larva paraffin section forming method, and belongs to the technical field of microscopic
tissue sections. The method comprises the following steps: fixing tissue, dehydrating the tissue, clearing the tissue, soaking with paraffin, embedding,
slicing, unfolding, pasting, removing paraffin, and rehydrating the tissue. The operation is simple, and the consumed time is short; the tissue distinction degree of sections is high finally, and the complete space structure of a
larva body can be observed; the method is suitable for
larva body materials which are soft in body, high in
liquid content and high in cavity proportion. When the method is adopted for
processing a three-year
cotton bollworm larva body, the body walls are complete when being observed at a bright field, and
muscle tissues, nerve tissues and the like in body cavities are distributed, a digestive duct structure, which is in a large ratio of the body, is clear and complete, and a pair of pleopods are normal in space structure; the excitated spontaneous green
fluorescence level of each organ tissue can be observed under
blue light excitation.