3 results about "Genotyping" patented technology

The invention provides a detection method for genotyping of human platelet alloantigen (HPA). The detection method is characterized in that a target fragment is obtained by designing a specific primerand then using a single-tube multiplex PCR amplification reaction; a single base extension primer is designed and then single base extension is performed; then a target DNA sequence is accurately measured by mass spectrometry. The platelet alloantigen HPA-1 to 21 bw allele can be detected at one time. Then a mass spectrum is typed directly, and data are directly output. A whole process is closed,the time is short, and the cost is low. Through detecting the genotyping of the platelet alloantigen of a patient and a blood donor, platelets matching successfully can be found, and the problem of ineffective platelet infusion caused by immune factors is effectively avoided. A detection kit product is low in cost and more accurate, and the detection method is expected to be a routine clinical detection method to replace a serological detection method.

The invention belongs to the field of molecular marker-assisted breeding of aquatic animals, and particularly relates to a group of prawn growth related SNP markers and an application thereof in breeding. The growth related markers are shown as sequences SNP3085_190487, SNP1678_860109, SNP3406_465799 and SNP2570_399791, the marked dominant genotypes of the growth related markers are CC, GG, GG andCC respectively, and the growth speed of prawns can be remarkably increased by performing genotyping and marker selection on one or more SNP markers. The invention also discloses an application of the growth related markers in genetic breeding. Compared with a traditional breeding method, the method has the advantages that character-related molecular markers are used for assisting in breeding, and accuracy and high efficiency are achieved; and besides, the multiple markers can be used in a combined mode, rapid improvement of growth characters is achieved through marker polymerization, and themethod has wide application prospects in cultivation of high-yield varieties.