3 results about "Heavy chain" patented technology

The present invention provides a monoclonal anti-idiotype antibody 11D10 that elicits an immune response against a specific epitope of a high molecular weight mucin of human milk fat globule (HMFG) and a hybridoma that produces 11D10. The hybridoma that produces 11D10 was selected by specific procedures. 11D10 induces an immunological response to HMFG in nice, rabbits, monkeys and patients with advanced HMFG-associated tumors. This invention provides compositions derived from polynucleotide sequences encoding the variable light and/or variable heavy regions of monoclonal anti-idiotype antibody 11D10, as well as polypeptides encoded thereby. The invention also provides compositions which can be used in the detection or treatment of HMFG-associated tumors.

The invention provides a monocytic leukemia associated antigen MLAA-34 resistant fully human monoclonal single-chain antibody ScFv. The monoclonal single-chain antibody ScFv of special anti-MLAA-34 is obtained by screening and identifying in a phage antibody library; MLAA-34 protein is firstly expressed and purified, and antigen protein is provided for monoclonal antibody preparation. A method beneficial to genetic engineering is used for screening and identifying anti-MLAA-34 fully human monoclonal antibody. The fully human monoclonal single-chain antibody ScFv for specially combining and efficiently expressing tumor cell strains of MLAA-34 is obtained. The fully human monoclonal single-chain antibody ScFv can be used for solving the immunogenicity problem of a mouse original antibody, and the single-chain antibody ScFv is formed by connecting a heavy chain and a light chain of an antibody variable region, is small in molecular weight, is high in penetrating power and is more beneficial to application.

Methods and materials are provided for decreasing or eliminating interference from molecules resulting from upstream immunochemical procedures, such as Immunoprecipitation, that employ an Immunoglobulin Binding Molecule (an IBM, e.g., Protein A) in subsequent downstream methods, such as Western Blot. An effective amount of a ligand of the IBM is used to block the IBM or fragments of the IBM on a support membrane used in the downstream method. In another embodiment, a secondary antibody that is specific either for heavy chain or light chain antibody fragments or that recognizes intact antibody molecules but does not recognize individual antibody fragments may be employed with the ligand of the IBM.