Method for decreasing interference in results of immunochemical methods

a technology of immunochemical methods and results, applied in the field of immunochemical analytical methods, can solve the problems of unfavorable useful data, unfavorable other products, and unfavorable multi-protein complexes in which a given target protein participates, and achieve the effect of a downstream technique from interfering molecules

Inactive Publication Date: 2007-08-02
BETHYL LAB
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Benefits of technology

The technical effect of this patented process involves reducing or removing certain materials during the course of chemistry experiments involving specific substances found within cells. This can be done through modifying the surface properties of supports made of these materials such as silica gel or glass fiber filters. By doing so, it becomes possible to isolate target compounds without affecting their function when analyzing them.

Problems solved by technology

Technologies described involve improving upon current technological processes involving detecting proteins called DNA probes during various steps involved in biomarkers assay operations. However, certain technical problem addressed by the present inventors relates to identifying smaller amounts of target substances without affecting the overall quality of the result due to interference caused by other materials like antigens. Existing solutions require expensive equipment and complicated process protocols, making interpretation difficult.

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  • Method for decreasing interference in results of immunochemical methods

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Embodiment Construction

[0022] Terms used herein have the following meanings:

[0023] An Immunoglobulin Binding Molecule (IBM) is a molecule that binds to immunoglobulins. Examples are Protein-A and Protein-G.

[0024] A Ligand of an Immunoglobulin Binding Molecule (LIBM) is any molecule that occupies the immunoglobulin binding site of an IBM or fragments thereof. An example is pig IgG or sheep IgG.

[0025] An upstream immunochemical procedure is one that: 1) uses immunoglobulins, 2) uses an IBM, and 3) produces a sample that is used in a downstream immunochemical procedure. An example is immunoprecipitation.

[0026] A downstream immunochemical procedure is a technique that uses immunoglobulins and is performed using a sample from an upstream immunochemical procedure. An example is Western Blotting.

[0027] A primary antibody is an immunoglobulin raised and selected based upon specific binding to a protein of interest. An example is an antibody raised in rabbits that recognizes the human protein known as histone de

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Abstract

Methods and materials are provided for decreasing or eliminating interference from molecules resulting from upstream immunochemical procedures, such as Immunoprecipitation, that employ an Immunoglobulin Binding Molecule (an IBM, e.g., Protein A) in subsequent downstream methods, such as Western Blot. An effective amount of a ligand of the IBM is used to block the IBM or fragments of the IBM on a support membrane used in the downstream method. In another embodiment, a secondary antibody that is specific either for heavy chain or light chain antibody fragments or that recognizes intact antibody molecules but does not recognize individual antibody fragments may be employed with the ligand of the IBM.

Description

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Claims

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Application Information

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Owner BETHYL LAB
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