The invention discloses a universal joint operation adjustable fixed operation channel, which comprises opening plates, movable supports and tissue clamps, the opening plates are semi-cylindrical, the two opening plates are symmetrically placed, the front sides and the rear sides of the two supporting plates are connected through the movable supports, and the tissue clamps are detachably mounted on the outer side walls of the opening plates; a threaded rod is arranged in the center of the movable support, a plurality of threaded rings are movably installed on the threaded rod, supporting rods are hinged to the left side and the right side of the threaded rod respectively, and the other ends of the supporting rods are hinged to the opening plate. The tissue clamp is formed by symmetrically installing two identical tissue clamps. A stable operation channel is arranged, the size of the channel can be adjusted, the application range is wide, operation time is saved, and risks are reduced; important tissues such as blood vessels, nerves, ligaments and tendons can be protected by the distraction plate, accidental injury in an operation is avoided, and the operation risk is reduced; the incision edge tissue is clamped through the two tissue clamps, tissue fluid leakage is reduced, the situation that the vision is affected by the soft tissue is avoided, and the operation efficiency is improved.