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2 results about "Mammary gland" patented technology

A mammary gland is an exocrine gland in humans and other mammals that produces milk to feed young offspring. Mammals get their name from the Latin word mamma, "breast". The mammary glands are arranged in organs such as the breasts in primates (for example, humans and chimpanzees), the udder in ruminants (for example, cows, goats, and deer), and the dugs of other animals (for example, dogs and cats). Lactorrhea, the occasional production of milk by the glands, can occur in any mammal, but in most mammals, lactation, the production of enough milk for nursing, occurs only in phenotypic females who have gestated in recent months or years. It is directed by hormonal guidance from sex steroids. In a few mammalian species, male lactation can occur. With humans male lactation can occur only under specific circumstances.

Method for targeted editing of buffalo 18S rDNA gene by virtue of adenovirus system

InactiveCN106591369AImprove editing efficiencyHigh titerNucleic acid vectorFermentation18s rdnaPrimary cell
The invention relates to the technical field of targeted editing of a buffalo 18S rDNA gene, in particular to a method for targeted editing of the buffalo 18S rDNA gene by virtue of an adenovirus system. According to the method provided by the invention, an autonomously constructed 18S gene editing vector is used for editing the buffalo 18S rDNA gene; a constructed adenovirus vector consists of Cas9 protein and sgRNA; and then, the vector is subjected to adenovirus packaging. The buffalo 18S rDNA gene is edited on the basis of the accurate and efficient performances of a Cas9 editing gene, a strong bearing capacity of adenovirus on exogenous genes as well as a strong infection capacity of the adenovirus on primary cells, so that a bottleneck that the buffalo primary cells are difficult for transfection; and the packaged adenovirus can efficiently infect buffalo primary mammary epithelial cells and can efficiently edit the buffalo 18S rDNA gene.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST

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