2 results about "Medical testing" patented technology

The invention relates to the field of medical checkup quality control, and concretely relates to a liquid composite protective agent for long-term culture preservation, and a preparation method and an application method thereof. The liquid composite protective agent for long-term culture preservation is prepared from the following components according to 100mL of solution agent: 15 to 20mL of ethylene glycol, 10 to 15mL of glycerinum, 0.5 to 1g of nutrient bouillon, 0.3 to 0.8g of trehalose, 0.1 to 0.5g of brain infusion powder, and the balance distilled water. The liquid composite protective agent for long-term culture preservation provided by the invention has good protection on various bacteria cultures, can ensure the activity of the cultures at the temperature being minus 20 DEG C and below after being mixed with the cultures, cannot affect the biochemical characters of microorganisms, has no need to be freeze-dried, redissolved and preserved in dark, and is convenient to use and operate in clinic bacterial detection. The cultures protected by the liquid composite protective agent provided by the invention can stably survive for more than 1 year at the temperature being minus 20 DEG C, and stably survive for more than 10 years at the temperature being minus 70 DEG C, so that great convenience is provided for the long-term preservation of special cultures.

The invention belongs to the field of medical examination, and relates to an in vivo antibacterial drug content determination method, in particular to an online solid phase extraction liquid chromatography for detecting content of linezolid in blood. The online solid phase extraction liquid chromatography for detecting the content of linezolid in the blood performs pretreatment on a to-be-detectedblood sample and then determines the content of linezolid by virtue of a high performance liquid chromatograph and by adopting a standard curve method. The online solid phase extraction liquid chromatography for detecting linezolid drug concentration in the blood has the advantages that blood plasma is subjected to protein precipitation and then is directly injected, operation of personnel is reduced and accuracy of a quantitative result is improved.