The invention provides a rosaceae cerasus plant ex-vitro rooting method, and relates to the technical field of tissue culture rooting. According to the rosaceae cerasus plant ex-vitro rooting method, a rooting environment in a test-tube plantlet bottle is simulated, so that tissue culture plantlets root in a relatively closed matrix with a nutrient solution, then the nutrient solution and a plastic film are gradually removed, and a matrix culture stage is directly carried out. According to the rosaceae cerasus plant ex-vitro rooting method, the rooting process and the transplanting process of tissue culture seedlings in the bottle are combined, so that the tissue culture seedlings directly root outside the bottle and in a transplanting medium, the production link is simplified, the production cost is reduced, and the emergence rate is increased. According to the rosaceae cerasus plant ex-vitro rooting method, ex-vitro rooting is carried out on the cerasus humilis tissue culture seedling, and the rooting rate can reach 80.6% at most.