1 results about "Spike Protein" patented technology

The invention relate to a recombinant baculovirus for expressing spike protein of chicken infectious bronchitis virus and application thereof, belonging to the biotechnical field. The recombinant baculovirus is prepared by a method comprising the following steps: obtaining S genes; building and indentifying a recombinant transfer vector pFastBacI-S; building and indentifying a recombinant shuttle vector AcIBV-SBacmid which infects Sf9 inset cells; and obtaining the recombinant baculovirus vAcIBV-S for expressing the spike protein of the chicken infectious bronchitis virus. The invention further provides the application of the recombinant baculovirus in the aspect of detecting chicken infectious bronchitis virus antibodies. The recombinant baculovirus can be used for detecting the chicken infectious bronchitis virus antibodies, thereby avoiding the risk of easy toxin diffusion when live virus serves as an antigen. The recombinant baculovirus can be used for detecting the chicken infectious bronchitis virus antibodies, can be used for quarantine on a large scale, and is especially suitable for use of quarantine departments at the basic level.