1 results about "Xanthine" patented technology

The invention discloses an improved culture medium for neonatal pig islet cells and a use method of the same. An apoptosis inhibitor Z-VAD-FMK, a human basic fibroblast growth factor hFGF-beta, a vascular endothelial growth factor VEGF, an insulin-like growth factor R3-IGF-1, a human epidermal growth factor hEGF, a hepatocyte growth factor hHGF, pig serum, sodium selenite, insulin, transferrin, ethanol amine, 1-methyl 3-isobutyl xanthine, nicotine, cortisol, penicillin and streptomycin are added in HAM'S F10 culture medium. According to the culture medium, apoptosis can be reduced and recovery rate can be increased; the cultured cells have a sensitive glucose stimulation response and more mature functions compared with generally-cultured islet cells; the insulin secretion amount of the islet cells with the same quantity is increased; the viability of islet beta-cells is improved; the breakages of islet cell clusters are reduced, and the envelops of the cell clusters are complete, so that the cell clusters are more suitable for being transplanted.