Method for confirming warfarin dose of Chinese population individuals

[0033] Embodiment: a method for determining the individual dosage of warfarin in the Chinese population, comprising the following steps:

[0034] (1) First, collect blood samples from the patient, then take 2ml of peripheral anticoagulant blood, use the Wizard(R) Genomic DNA Purification Kit, and extract DNA in strict accordance with the kit instructions;

[0035] (2) Perform PCR amplification on the extracted genomic DNA; PCR reaction system volume is 25 μl, genomic DNA 0.2 μg, 10XPCR buffer 2.5 μl, 2.5mmol / L dNTP 2μl, 25mmol / LMgcl 2 1.5 μl, primer P 1 10pmol, primer P 2 10pmol, Taq polymerase (5U / L) 0.15μl; PCR reaction conditions: 95°C pre-denaturation for 5min, 94°C for 30s, 59°C for 20s, 72°C for 30s, a total of 35 cycles, 72°C extension for 7min. VKORC1 gene forward primer is 5′-CCAGCAGGAGAGGGAAATA-3′, reverse primer is 5′-TTTGG-ACTACAGGTGCCT-3′; CYP2C9 gene forward primer is 5′-TGCACGAGGTCCAGAGATGC-3′, reverse primer is 5′-AAACATGGAGTTGCAGTGTAG -3', the primer