Cultivation method of triploid fries of haliotis discuses

A wrinkled plate abalone and its cultivation method technology, which is applied in the field of abalone, can solve the problems of slow growth and reduced survival rate of wrinkled plate abalone, and achieve good economic benefits, fast growth and rapid growth

Inactive Publication Date: 2011-07-27
郭炳坚
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problems of slow growth rate and decreased survival rate of abalone in the breeding season in the existing b

Method used

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Effect test

Embodiment 1

[0016] 1) Before the maturation of the gonads of the rugosa abalone, the rugosa abalone with a shell length ranging from 7 to 12 cm, good individual vitality and no muscle damage was selected as the seed abalone, and artificial cultivation was carried out to make the gonads of all the abalones mature at the same time.

[0017] 2) Stimulate the seed abalone by artificial oxytocin, induce the seed abalone to oviposit or ejaculate, and collect the sperm and eggs respectively. Artificial insemination was carried out within 15 minutes after sperm production and within 40 minutes after egg production, and the male to female ratio of the species involved in the insemination was 30:1. After fertilization, quickly use a 300-mesh sieve tulle bag to concentrate the fertilized eggs to 5 million grains / L.

[0018] 3) When the first polar body is observed in 30% to 50% of the fertilized eggs under the optical microscope, the triploid induction is started on the fertilized eggs, and the sieve

Embodiment 2

[0021] 1) Before the maturation of the gonads of the rugosa abalone, the rugosa abalone with a shell length ranging from 7 to 12 cm, good individual vitality and no muscle damage was selected as the seed abalone, and artificial cultivation was carried out to make the gonads of all the abalones mature at the same time.

[0022] 2) Stimulate the seed abalone by artificial oxytocin, induce the seed abalone to oviposit or ejaculate, and collect the sperm and eggs respectively. Artificial insemination was carried out within 20 minutes after sperm production and within 50 minutes after egg production, and the male to female ratio of abalones participating in the insemination was 40:1. After fertilization, quickly use a 300-mesh sieve tulle bag to concentrate the fertilized eggs to 8 million grains / L.

[0023] 3) When the first polar body is observed in 30% to 50% of the fertilized eggs under the optical microscope, triploid induction is carried out on the fertilized eggs, and the sea

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Abstract

The invention provides a cultivation method of triploid fries of haliotis discuses and relates to abalone. The cultivation method comprises the following steps of: selecting haliotis discuses which have the shell length in the range of 7-12cm, have good individual vitality, and have no damage on muscles as parent abalones, developing artificial feeding on the parent abalones, and ensuring the synchronous maturity of the gonadal development of the parent abalones through the artificial feeding; simulating the parent abalones by adopting an artificial propagation method, respectively inducing the parent abalones to produce milt and lay eggs, collecting the milt and eggs for artificial insemination, and obtaining fertilized eggs of the haliotis discuses; and washing the fertilized eggs of the haliotis discuses for 10-15min through seawater with the temperature of 2-3 DEG C based on the time of observing 30%-50% of the fertilized eggs of the haliotis discuses to produce first polar bodies under an optical microscope as starting time. A ploidy analyzer is adopted to detect the triploid rate of cultured fries of the haliotis discuses after the low temperature induction. When the cultivation method is adopted, the triploid fries of haliotis discuses characterized in excellent quality, rapid growth, large body, high survival rate and the like can be cultivated in a large scale, and the cultivation method has good economic benefit.

Description

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Claims

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Application Information

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Owner 郭炳坚
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