Treatment method of contaminated water body by promoting degrading bacteria to be fixedly planted at biological film

[0024] (1) Cultivation of coaggregating bacteria (bridging bacteria): take the bridging bacteria that have been isolated and screened in advance and have extensive coaggregation ability Bacillus cereus G5, for the separation and screening method, please refer to the document "Research on the Coaggregation of Bacteria in the Biofilm of Wastewater Treatment System" ([J] Anhui Agricultural Sciences, 2010, 38(11): 5752-5754). After activation culture, it is inoculated into cross-bridging bacteria culture Incubate for 20 hours at 30°C, 150 rpm, and centrifuge to collect the bacteria. The formula of the bridging bacteria culture solution is that each 1L of tap water includes: peptone 5 g, yeast powder 5 g, (NH 4 ) 2 NO 3 1.5 g, NaCl 0.1 g, MgSO 4 ·7H 2 O 0.2 g; CaCl 2 ·2H 2 O 0.05 g, FeCl 3 ·6H 2 O 0.01 g, K 2 HPO 4 0.5 g, adjust pH=7.2.

[0025] (2) Cultivation of degrading bacteria: Degrading bacteria can choose to use a variety of degrading bacteria that have been reported or obtain

[0031] (1) Cultivation of coaggregating bacteria: According to the technical scheme of Example 1, bridging bacteria with extensive coaggregation ability were isolated and screened Bacillus megaterium T1: After activation and culture, it is inoculated into the cross-bredging bacteria culture solution, cultured at 30°C, 150 rpm for 20 hours, and centrifuged to collect the bacteria. The formula of bridging bacteria culture solution includes: peptone 5 g, yeast powder 2 g, (NH 4 ) 2 NO 3 1.5 g, NaCl 0.1 g, MgSO 4 ·7H 2 O 0.2 g; CaCl 2 ·2H 2 O 0.05 g, FeCl 3 ·6H 2 O 0.01 g, K 2 HPO 4 0.5 g, 1L tap water, adjust pH=7.4.

[0032] (2) Cultivation of degrading bacteria: taking phenol degrading bacteria Pseudomonas sp. FY-6, refer to the literature "Preliminary identification of phenol-degrading strain FY-6 and research on phenol-degrading characteristics" ([J] Bulletin of Science and Technology, 2009, 25(4): 441-444), after activation culture Inoculate into the degrading bacteria cultur

[0036] (1) According to the conventional microbiology experiment method, streak inoculate the bacteria and degrading bacteria with extensive coagglutination ability obtained in advance to the slope of the LB test tube and incubate at 30°C for 24 hours.

[0037] (2) Pick the bacteria on the test tube and inoculate it into a 250 mL Erlenmeyer flask containing 100 mL of LB liquid medium, and culture it with shaking at 30°C and 150 rpm for 24 hours.

[0038] (3) Inoculate the strains in the Erlenmeyer flask into a 1000 mL Erlenmeyer flask containing 300 mL of LB liquid medium according to the 10% inoculum volume, and culture at 30°C, 150 revolutions / min with shaking for 24 hours.

[0039] (4) Collect the bacterial suspensions of two kinds of bacteria, centrifuge at 6000 rpm, and use 2 mmol CaCl 2 ·2H 2 O and 2 mmol MgSO 4 ·7H 2 Omol / L 0.1 phosphate buffer (pH 7.0) is made into OD 660 =1 bacterial suspension, the two bacterial suspensions are mixed in a ratio of 1:1, and left to stand for