Preparation method of targeted ferroferric oxide-porphyrin containing composite nanoparticles

A technology of composite nanoparticles and ferric oxide, which is applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problem of weak fluorescence signal intensity of photosensitizers and tumor tissue selection. The problem of low sexual uptake and high cumulative effect of photosensitizers can achieve the effect of inhibiting tumor metastasis, improving immunity and reducing diffusion

Inactive Publication Date: 2014-02-05
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The present invention aims to solve the problems of photodynamic diagnosis and treatment that the fluorescent signal intensity of the photosensitizer is not strong, and the selective uptake of tumor tissue is not high, resulting in a high

Method used

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Example Embodiment

[0026] Specific embodiment 1: This embodiment has a method for preparing targeted ferroferric oxide-porphyrin composite nanoparticles, which is specifically carried out according to the following steps:

[0027] 1. Using FeCl 3 ·6H 2 O prepares FeCl with a concentration of 0.1 mol / L 3 Solution using FeSO 4 ·4H 2 O prepares FeSO with a concentration of 0.1 mol / L 4 Solution

[0028] Under nitrogen protection and mechanical stirring conditions, according to Fe 3+ With Fe 2+ The molar ratio is 2:1, and 100mL FeCl with a concentration of 0.1mol / L 3 Solution and 50mL FeSO with a concentration of 0.1mol / L 4 Mix the solution to get FeCl 3 And FeSO 4 Then, use hydrochloric acid with a concentration of 1mol / L to adjust the pH to 5, and then add an aqueous ammonia solution with a mass concentration of 25% until the mixed solution appears black precipitation, and then continue to stir for 1h to obtain a solid-liquid mixture;

[0029] Under the action of an external magnetic field, the uppe

Example Embodiment

[0035] Specific embodiment two: This embodiment is different from specific embodiment one in that step one uses a mixture of twice distilled water and ethanol with a volume ratio of twice distilled water and ethanol of 1:1 for washing. Others are the same as the first embodiment.

Example Embodiment

[0036] Specific embodiment three: This embodiment is different from specific embodiment one in that 100 mL of double distilled water is added in step two. Others are the same as the first embodiment.

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Abstract

The invention discloses a preparation method of targeted ferroferric oxide-porphyrin containing composite nanoparticles, and relates to a preparation method of composite nanoparticles. The preparation method disclosed by the invention aims at solving the problems that a photosensitizer is high in accumulation effect within normal tissues and even performs a photochemical reaction under a dark light because a photosensitizer fluorescence signal is low in intensity and low in selective uptake for tumour tissues, existing in photodynamic diagnosis and therapy. The method comprises the following steps: 1, preparing magnetic ferroferric oxide nanoparticles coated by silicon dioxide; 2, preparing a porphyrin compound; 3, preparing a porphyrin compound bound by a silane reagent; 4, preparing a porphyrin-folic acid compound; and 5, preparing the targeted ferroferric oxide-porphyrin containing composite nanoparticles. The preparation method based on the magnetic ferroferric oxide-porphyrin composite nanoparticles and disclosed by the invention integrates magnetofluid thermal therapy, magnetic targeting and photodynamic therapy as a therapy means for tumours. The preparation method disclosed by the invention is used for preparing the targeted ferroferric oxide-porphyrin containing composite nanoparticles.

Description

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Claims

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Application Information

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Owner HARBIN INST OF TECH
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