Ratio type fluorescence probe for determining CN<-> as well as preparation method and application thereof
A fluorescent probe and ratio-based technology, applied in the field of fluorescent probes, can solve the problems of expensive raw materials and complicated preparation, and achieve the effects of simple reaction conditions, simple detection methods, and good cell membrane penetration.
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[0024] Example 1 Determination of CN - Of the ratio-type fluorescent probe Probe1
[0025] Dissolve 0.223g (1mmol) N-ethylcarbazole-3-carbaldehyde and 0.365g (1mmol) 1,1,2-trimethyl-3-ethylbenzindole iodide in 10mL ethanol and heat to reflux React for 4 hours, cool, filter, and wash with ethanol to obtain 0.34 g of purple-brown solid, with a yield of 59.6%.
[0026] For Probe1 1 HNMR characterization, the results are as follows:
[0027] 1 HNMR(300MHz, CDCl 3 ): δ8.20(d,J=8.4Hz,3H), 8.03(d,J=7.5Hz,1H), 7.91(d,J=15.3Hz,2H), 7.73(t,J=14.7Hz,1H ), 7.64 (t, J = 14.7 Hz, 1H), 7.56 (q, J = 25.5 Hz, 1H), 7.39 (m, J = 27.3 Hz, 3H), 7.27 (s, 2H), 5.12 (d, J =6.0Hz,2H), 4.29(d,J=7.2Hz,2H),2.09(s,2H),1.65(s,3H),1.37(t,J=14.1Hz,3H)
[0028] 13 CNMR(75MHz, CDCl 3 ): δ156.66,149.67,144.63,141.10,141.29,138.42,134.01,132.20,131.18,129.18,128.11,126.10,125.02,123.84,121.73,112.36,110.59,109.93,108.62,54.09,44.51,38.79,16.03, 15.16
Example Embodiment
[0029] Example 2 CN of Fluorescent Probe Probe1 - Titration fluorogram
[0030] Add 20μL fluorescent probe Probe1 stock solution to 2mL DMSO system for CN - Fluorescence titration experiment was tested on a fluorescence spectrophotometer. With the addition of the sample to be tested, the fluorescence intensity at 440nm gradually increased, and the fluorescence intensity at 595nm gradually increased. Instrument parameters: The slit widths of excitation wavelength and emission wavelength are 2.5nm and 5.0nm respectively, and the maximum excitation wavelength of the fluorescent probe solution is: λ ex Is 340nm and the maximum fluorescence emission wavelength is: λ em It is 595nm.
Example Embodiment
[0031] Example 3 CN of Fluorescent Probe Probe1 - Titration UV chart
[0032] Add 20μL fluorescent probe Probe1 stock solution to 2mL DMSO system for CN - Ultraviolet titration experiment, detected on an ultraviolet spectrophotometer, with the addition of the sample to be tested, the ultraviolet absorption intensity at 503nm gradually weakened, and the ultraviolet absorption peak intensity at 258nm and 285nm gradually increased.
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