Enterovirus 71 detection method and detection kit

[0030] 1. Preparation of Polyclonal Antibody

[0031] 1.1 Preparation of immunogen

[0032] 1.1.1 Design of EV71VP1 gene sequence primers

[0033] According to the EV71 capsid protein VP1 gene sequence queried in GenBank (accession number: EU024958.1), primers were designed by ourselves. '-CCGCTCGAGGAGDGTGGTGATRGCRGTGCGACT-3'.

[0034] 1.1.2 RNA extraction of EV71 virus

[0035] Extract the RNA of EV71 virus (gifted by Zhejiang CDC) as a template:

[0036]Use the viral RNA extraction kit from Roche Company in the United States to directly extract RNA from the cultured virus. The operation steps are carried out according to the instructions in the kit. The method is as follows:

[0037] 1) Take a 1.5mL centrifuge tube, mark it and add 409μL of the working solution that has been mixed;

[0038] 2) Add 200 μL of the sample to be extracted with an RNase-free tip with a filter element, shake and mix on a vortex mixer, centrifuge briefly, and let stand at room temperature for 10 m

[0088] 1.1 Use the prepared kit for sample detection