Bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue and preparation method thereof

[0092] Based on the preparation method of bladder acellular matrix-cell-three-dimensional fiber network biomimetic tissue, the acellular matrix material from the bladder is prepared, freeze-dried, then freeze-ground, crushed into powder; digested with pepsin in hydrochloric acid solution Bladder decellularized matrix powder for about 48 hours to form bladder decellularized matrix pregel solution, add about 1 / 10 of the volume of bladder decellularized matrix pregel solution NaOH, wherein the concentration of NaOH is 0.01mol / L, then add about 1 / 10 9 volumes of bladder acellular matrix pregel solution volume 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add insulin, wherein the mass ratio of insulin to bladder acellular matrix is ​​0.05:100, to form bladder acellular matrix gel precursor solution. Before the gel was formed, the above-mentioned bladder acellular matrix precursor solution was coated on the fibers prepared by the three-dimensional weaving method w

[0094] Based on the preparation method of bladder acellular matrix-cell-three-dimensional fiber network biomimetic tissue, the acellular matrix material from the bladder is prepared, freeze-dried, then freeze-ground, crushed into powder; digested with pepsin in hydrochloric acid solution Bladder decellularized matrix powder for about 48 hours to form bladder decellularized matrix pregel solution, add about 1 / 10 of the volume of bladder decellularized matrix pregel solution NaOH, wherein the concentration of NaOH is 0.01mol / L, then add about 1 / 10 9 volumes of bladder acellular matrix pregel solution volume 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add aspirin, wherein the mass ratio of aspirin to bladder acellular matrix is ​​1:20, to form bladder acellular matrix gel precursor solution. Before the gel was formed, the above-mentioned bladder acellular matrix precursor solution was sprayed on the fibers prepared by the three-dimensional weaving method with

[0096] Preparation method of biomimetic tissue based on bladder acellular matrix-cell-three-dimensional fiber network: prepare the acellular matrix material from the bladder, freeze-dry it, freeze and grind it, and break it into powder; digest it with pepsin in hydrochloric acid solution The acellular matrix powder is about 48 hours to form a bladder acellular matrix pregel solution, add about 1 / 10 of the volume of the bladder acellular matrix pregel solution NaOH, wherein the concentration of NaOH is 0.01mol / L, and then add about 1 / 9 Bladder decellularized matrix pregel solution volume 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add ketoprofen, wherein the mass ratio of ketoprofen to bladder decellularized matrix is ​​1:10, to form decellularized bladder Matrix gel precursor solution. Before the gel was formed, the above-mentioned bladder acellular matrix precursor solution was coated on the fibers prepared by the three-dimensional weaving method with a d