Vein-iodine rapid detection and diagnosis method for citrus yellow shoot
A technology of citrus huanglongbing and diagnosis method, which is applied in the field of rapid detection and diagnosis of citrus huanglongbing-iodine tincture, which can solve the problems of low accuracy, high cost, and high detection technology requirements, and achieve high accuracy, convenient detection, and easy material collection Effect
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Embodiment 1
[0027] The leaf vein-iodine tincture rapid detection and diagnosis method of citrus huanglongbing comprises the following steps:
[0028] 1) Sampling: random collection needs to detect mature leaves of citrus plants;
[0029] 2) Scrape off the epidermis of the leaf midrib with a blade in a balanced manner to expose the phloem;
[0030] 3) Apply 1.5% tincture of iodine evenly on the midvein phloem with a cotton swab;
[0031] 4) Results observation and diagnosis: Wait for 50 seconds to 3 minutes; if the midrib turns blue, it is positive, which means citrus huanglongbing;
Embodiment 2
[0033] The leaf vein-iodine tincture rapid detection and diagnosis method of citrus huanglongbing comprises the following steps:
[0034] 1) Sampling: random collection needs to detect mature leaves of citrus plants;
[0035] 2) Scrape off the epidermis of the leaf midrib with a blade in a balanced manner to expose the phloem;
[0036] 3) Apply 5.0% tincture of iodine evenly on the midvein phloem with a cotton swab;
[0037] 4) Results observation and diagnosis: Wait for 50 seconds to 3 minutes; if the midrib turns blue, it is positive, which means citrus huanglongbing;
Embodiment 3
[0039] The leaf vein-iodine tincture rapid detection and diagnosis method of citrus huanglongbing comprises the following steps:
[0040] 1) Sampling: random collection needs to detect mature leaves of citrus plants;
[0041] 2) Scrape off the epidermis of the leaf midrib with a blade in a balanced manner to expose the phloem;
[0042] 3) Apply 2.0% tincture of iodine evenly on the midvein phloem with a cotton swab;
[0043] 4) Results observation and diagnosis: Wait for 50 seconds to 3 minutes; if the midrib turns blue, it is positive, which means citrus huanglongbing;
[0044] Table 1 Detection status of citrus Huanglongbing
[0045]
[0046]
[0047] Result: the applicant adopts the method described in the present invention to detect 989 times (see Table 1) to the citrus samples of grounds such as Qinzhou, Fangcheng, Dongxing through more than 3 years, image 3 On the left is the test result of one of the samples: the midvein turns blue and is positive, which is citru
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