Flowering day lily extract, preparation method and usage of flowering day lily extract, and antidepressant drug combination

[0048] Example 1 Preparation of Water Extract of Flowering Daylily

[0049] (1) Grind the flowering daylily, add 8 times the mass of water at room temperature to extract for 6 hours, and concentrate the extract after extraction to obtain the first extract;

[0050] (2) Continue to add 8 times the mass of room temperature water to the residue to extract for 3 hours, and concentrate the extract after extraction to obtain the second extract;

[0051] (3) Combine the first extract and the second extract to obtain the third extract, and freeze the third extract at -80°C for 24 hours;

[0052] (4) The frozen third extract is freeze-dried for 72 hours, then taken, pulverized and powdered to prepare an aqueous extract of flowering daylily.

[0053] Example 2 Preparation of Alcohol Extract of Flowering Daylily

[0054] (1) Grind the flowering daylily, add 8 times the mass of ethanol with a volume concentration of 85% to extract for 6 hours, and concentrate the extract after extraction to obtain the first extract;

[0055] (2) Continue to add 8 times the mass of ethanol with a volume concentration of 85% to the residue for 3 hours, and then concentrate the extract to obtain the second extract;

[0056] (3) Combine the first extract and the second extract to obtain the third extract, and freeze the third extract at -80°C for 24 hours;

[0057] (4) The frozen third extract is freeze-dried for 72 hours, then pulverized and powdered to obtain an alcohol extract of flowering daylily.

[0058] Figure 4 In order to prepare the total ion current diagrams of the aqueous extract of flowering day lily and the ethanol extract of flowering day lily, it can be seen that the composition of the two extracts is different to a certain extent.

[0059] Example 3 Effect of day lily extract on mouse depression model caused by chronic unpredictable stress

[0060] (1) Establishment of a mouse depression model caused by chronic unpredictable stress

[0061] 140 male ICR mice, weighing 18.0-22.0g, except for the 10 normal control group, the remaining mice were subjected to chronic unpredictable stress, including fasting (12h), water deprivation (12h), and forced swimming (10min) , Strobe (12h), noise (30min), restraint (12h) (placed in a 50ml centrifuge tube, the tube diameter is 3.0cm, the length is about 10cm, the tube wall has 6-7 vents with a diameter of 0.5mm), tilt Cage (12h), wet cage (12h), day and night upside down, etc. Animals are fasted and water-free during the restraint period.

[0062] Table 1 Chronic unpredictable stimulation schedule

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[0065] (2) Grouping and administration

[0066] After 28 days of continuous modeling, the animals were randomly grouped according to the test results of sugar and wat