Preparation method of zif-8 nanosphere loaded SERS coded gold nanoparticles for intracellular photothermal therapy

[0038] (1) Utilize the classical method sodium citrate reduction method described in the literature to synthesize 13nm gold nanoparticles, simply, put 0.01%, 300mL chloroauric acid in a 500mL round bottom flask with a reflux condenser, stir and add after vigorous boiling 1%, 9mL trisodium citrate aqueous solution, react for 20min, until the solution turns red, rapidly cool the obtained gold nanoparticle solution with ice water, and set aside;

[0039] (2) Take 100mL of the gold nanoparticles in (1) in a beaker, and configure a 3 μmol / L Raman reporter DTTC solution, take 10mL dropwise and add it to the stirred gold nanoparticle solution, and react for 10min to obtain a Raman Reporter DTTC-labeled gold nanoparticles;

[0040] (3) Take 50mL (2) of Raman reporter DTTC-labeled gold nanoparticles, add 3μmol / L polyethylene glycol mPEG-SH, 10mL, 3μmol / L HOOC-PEG-SH, 10mL, react for 2h, centrifuge at 16000rpm Collect the precipitate, disperse and wash it with deionized water for 3

[0045] (1) Utilize the classical method sodium citrate reduction method described in the literature to synthesize 13nm gold nanoparticles, simply, put 0.01%, 300mL chloroauric acid in a 500mL round bottom flask with a reflux condenser, stir and add after vigorous boiling 1%, 9mL trisodium citrate aqueous solution, react for 20min, until the solution turns red, rapidly cool the obtained gold nanoparticle solution with ice water, and set aside;

[0046] (2) Take 200mL of the gold nanoparticles in (1) in a beaker, and configure a 3 μmol / L Raman reporter DTTC solution, take 50mL dropwise and add it to the stirred gold nanoparticle solution, and react for 10min to obtain a Raman Reporter DTTC-labeled gold nanoparticles;

[0047] (3) Take 100mL (2) of Raman reporter DTTC-labeled gold nanoparticles, add 5μmol / L polyethylene glycol mPEG-SH, 10mL, 5μmol / L HOOC-PEG-SH, 10mL, react for 2h, centrifuge at 16000rpm Collect the precipitate, disperse and wash it with deionized water for 3

[0052] (1) Utilize the classical method sodium citrate reduction method described in the literature to synthesize 13nm gold nanoparticles, simply, put 0.01%, 300mL chloroauric acid in a 500mL round bottom flask with a reflux condenser, stir and add after vigorous boiling 1%, 9mL trisodium citrate aqueous solution, react for 20min, until the solution turns red, rapidly cool the obtained gold nanoparticle solution with ice water, and set aside;

[0053] (2) Take 1000mL of the gold nanoparticles in (1) in a beaker, and configure a 3 μmol / L Raman reporter DTTC solution, take 100mL and add it dropwise to the stirred gold nanoparticle solution, and react for 10min to obtain a Raman Reporter DTTC-labeled gold nanoparticles;

[0054] (3) Take 200mL (2) of Raman reporter DTTC-labeled gold nanoparticles, add 10μmol / L polyethylene glycol mPEG-SH, 10mL, 10μmol / L HOOC-PEG-SH, 10mL, react for 2h, 16000 rpm The precipitate was collected by centrifugation, dispersed and washed 3 times wit