Preparation process of odd-number carrageenan oligosaccharide
A preparation process and carrageenan technology, which are applied in the preparation of sugar derivatives, oligosaccharides, sugar derivatives, etc., can solve the problem that the preparation method of carrageenan odd-numbered oligosaccharides is not clear, and the production problems cannot be well solved. It can save labor and time costs, save use and storage costs, and reduce production pollution.
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Embodiment 1
[0039] A kind of preparation technology of carrageenan odd number oligosaccharide, described preparation technology comprises the following steps:
[0040] 1) the purification of κ-carrageenan, it comprises the following steps:
[0041] ① Take 1kg of kappa-carrageenan raw material and make 1% glue solution with distilled water, heat to 70°C to dissolve and filter while hot to obtain the filtrate;
[0042] 2. Add an equal volume of KCl solution to the above-mentioned step 1. gained filtrate, while constantly stirring, the concentration of the KCl solution is 0.25mol / L;
[0043] ③ Place the solution obtained in the above step ② at room temperature for 4 hours, then perform suction filtration to collect the precipitate;
[0044] ④ Add about 1.5 times the amount of 75% ethanol to the precipitate obtained in the above step ③ while stirring continuously, let it stand for 30 minutes, then collect the precipitate by suction filtration, and then dehydrate the precipitate with absolute
Embodiment 2
[0055] The present invention also introduces another preparation process of carrageenan acid-hydrolyzed odd-numbered oligosaccharides, which is the same as that of Example 1, except for two differences. First, the acid hydrolysis of κ-carrageenan uses 0.1 mol / L TFA for acid hydrolysis; second, the alkalization treatment uses KOH to adjust the pH.
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