Preparation method of biomedical tissue adhesive

[0022] Example 1

[0023] (1) Preparation of the mass fraction of 20% aqueous solution of dextran, with the mass fraction of 1% aqueous solution of sodium periodate mixing, both control a volume ratio of 2: 1, and finally at a constant temperature of 30 ℃ 60min; after completion of the reaction, the mixture was subjected to dialysis with running water for 18h, followed by a mixture of deionized water twice further dialyzed, the dialysis time of 1.5h, and finally by drying to obtain dextran aldehyde which was ground to a powder vacuum dried for 24h, stand refrigerated at -15 deg.] C;

[0024] (2) preparing a mass fraction of 25% aqueous solution of ε- polylysine, acetic anhydride with deionized water to a concentration of 6% aqueous solution of acetic anhydride, the volume ratio of the two solutions were 2: 1 mixture, at a constant temperature stirring the reaction at 45 ℃ 60min, after the completion of the reaction, deionized water, the reaction solution was purified by dialysis for

[0026] Example 2

[0027] (1) Preparation of the mass fraction of 20% aqueous solution of dextran, with the mass fraction of 1% aqueous solution of sodium periodate mixing, both control a volume ratio of 2: 1, and finally at a constant temperature of 70 ℃ 60min; after completion of the reaction, the mixture was subjected to dialysis with running water for 18h, followed by a mixture of deionized water twice further dialyzed, the dialysis time of 1.5h, and finally by drying to obtain dextran aldehyde which was ground to a powder vacuum dried for 24h, stand refrigerated at -15 deg.] C;

[0028] (2) preparing a mass fraction of 25% aqueous solution of ε- polylysine, acetic anhydride with deionized water to a concentration of 6% aqueous solution of acetic anhydride, the volume ratio of the two solutions were 2: 1 mixture, at a constant temperature stirring the reaction at 45 ℃ 60min, after the completion of the reaction, deionized water, the reaction solution was purified by dialysis for

[0030] Example 3

[0031] (1) Preparation of the mass fraction of 20% aqueous solution of dextran, from the weight fraction of 20% aqueous solution of sodium periodate mixing, both control a volume ratio of 2: 1, and finally at a constant temperature of 30 ℃ 60min; after completion of the reaction, the mixture was subjected to dialysis with running water for 18h, followed by a mixture of deionized water twice further dialyzed, the dialysis time of 1.5h, and finally by drying to obtain dextran aldehyde which was ground to a powder vacuum dried for 24h, stand refrigerated at -15 deg.] C;

[0032] (2) preparing a mass fraction of 25% aqueous solution of ε- polylysine, acetic anhydride with deionized water to a concentration of 6% aqueous solution of acetic anhydride, the volume ratio of the two solutions were 2: 1 mixture, at a constant temperature stirring the reaction at 45 ℃ 60min, after the completion of the reaction, deionized water, the reaction solution was purified by dialysis f