Discrimination of MUC4 mononucleotide polymorphic site with obvious effect on diarrhea resistance of weaned piglet and its application

[0029]The pig resource family constructed by itself, with white Duroc and Chinese Erhualian pigs as ancestral parents and used for functional gene mapping, is used as experimental animals. 795 boars of the second generation (F2) of the resource family were raised to 240 days of age and slaughtered. The small intestine and jejunum were taken, and the brush border of small intestinal epithelial cells was extracted. E.coli F4ab, F4ac and F4ad strains were used to adhere to the porcine small intestinal epithelial cell brush. Like edge, the result of bacterial adhesion is detected by phase-contrast microscope. Each sample detects more than 20 brush borders. If more than 10% of the brush borders have at least 2 bacteria attached, the sample is judged to be an adhesive type. If there are at least 2 bacteria attached to the brush borders less than 10 %, but there are more than 10% of the brush-shaped border attached by 1-2 bacteria, the sample is judged to be a weak adhesion type, otherwise