The invention discloses a fresh-keeping processing and packaging process for berries like strawberries and blueberry berries. The process comprises the following steps: A) removing bad and rotten fruits from fresh berries, carefully placing the fruits in a tray or a basket, and precooling the fruits in an air-cooled cold storage at 0-2 DEG C for 24 hours, until temperature at the fruit center drops to 2 DEG C, while introducing O3 concentration of 3ppm into the cold storage for 15 min; B) placing the cooled berry fruits into a grid made of a soft material, wherein aperture of the grid suits the size of a single berry fruit; and C) placing the grid and the berry fruits into a sterile polypropylene box, and packaging the fruits by a controlled atmosphere packaging method. According to the above process provided by the invention, a fresh-keeping period of the strawberries or blueberry berries is prolonged from an original 2 days to 15 days, without loss of appearance, color or taste of the fresh fruits.