The invention discloses pumpkin root honey yogurt with an effect of promoting lactation and a preparation method thereof. The pumpkin root honey yogurt comprises the following raw material components in parts by weight: 180-220 parts of fresh milk, 20-30 parts of red core dragon fruit, 0.02-0.05 part of pectinase, 13-17 parts of honey, 7-9 parts of cane sugar, 4-6 parts of yogurt bacterial powder, 1-3 parts of gelatin, 1-3 parts of crucian carp, 2-4 parts of pumpkin roots, 1-3 parts of juicy peach, 2-4 parts of apples and 1-3 parts of wax gourd. The honey yogurt prepared by the process disclosed by the invention is good in flavor and good in taste, and the pumpkin root and crucian carp serve as raw materials, so that the prepared yogurt has the health effects of promoting lactation and the like and is suitable for lying-in woman.