1 results about "Epidemiology" patented technology

The invention belongs to the technical field of nucleic acid detection, and discloses a primer combination and a kit for identifying an African swine fever virus gene deletion strain and an African swine fever epidemic strain by using a centrifugal microfluidic chip. The kit comprises a microfluidic chip, an isothermal amplification premixed solution, an isothermal amplification enzyme solution and a positive standard. The invention further relates to a detection method adopting the kit. The detection method comprises the steps of coating of a primer composition, extraction of a nucleic acid sample to be detected, LAMP reaction and result interpretation. The detection system can rapidly, conveniently, efficiently, specifically and sensitively identify and diagnose African swine fever gene deletion strain and African swine fever wild strain under isothermal conditions, does not need expensive and complex instruments, provides a novel portable detection platform for epidemiological detection and prevention and control of African swine fever, greatly reduces detection cost, is easy to popularize in a large range, and has broad market prospects.