The invention discloses an angle steel-gusset plate connection node semi-rigidity detection method in a power transmission angle steel tower structure. The method comprises the following steps that: S10, angle steel is installed on a gusset plate to obtain multiple groups of measuring pieces, wherein the measuring pieces are numbered, the angle steel and the gusset plate are made of Q420 steel, and bolts are 8.8-level or 10.9-level high-strength bolts of M20; S20, the measuring pieces are mounted on the testing device, and a measuring scheme is made; S30, the pressurization test is conducted on the test piece through a test device to obtain corner data of an angle steel shearing domain and a node domain; S40, the tensile test on each angle steel is carried out to obtain ultimate tensile stress, elastic modulus, yield stress and yield strain, wherein test piece sampling position of the angle steel is at a position which is 1/3 away from the limb width in the longitudinal direction of the angle steel; S50, the bearing capacity of the node plate, the local shearing corner of the angle steel and the bending moment bearing capacity of the node are is detected; and S60, the test structure of the angle steel-gusset plate is analyzed. The method has the advantage of accurate measurement data.