The invention discloses an in-vitro heart constant pressure/constant flow perfusion experiment device for cardiological mammals. The device comprises a fixing and supporting device, a perfusion device and a monitoring device. The fixing and supporting device comprises a bottom platform. A fixed supporting plate and a movable supporting plate are arranged at the rear side of the bottom platform. A hydraulic lifter is arranged below the movable supporting plate. The perfusion device comprises a baffling perfusion column, and the baffling perfusion column is fixedly arranged on the front face of the movable supporting plate. The bottom of the baffling perfusion column is connected with the heart, and a conical incubator and a blood filter are sequentially arranged below the heat. The right side of the blood filter is connected with an oxygenator, and the oxygenator is connected with a gas storage bottle, a liquid storage bottle and a peristaltic pump through a gas conveying pipe, a liquid replenishing pipe and a liquid conveying pipe, respectively. The tail end of the liquid conveying pipe is connected with a liquid inlet of the baffling perfusion column. The baffling perfusion column and the conical incubator are connected with a temperature controller by means of a manifold. The monitoring device collects and records the test data through an electrode, a balloon, a heart sound transducer and a pressure transducer.