The invention relates to a freezing gun for puncture type cryotherapy. The freezing gun comprises a shell, a hydraulic transmission mechanism, a liquid storage container, a button and a plurality of freezing heads. One end of the hydraulic transmission mechanism is connected with the button, and the other end of the hydraulic transmission mechanism is connected with the liquid storage container; by pressing the button, the thrust of a syringe needle on the liquid storage container is effectively increased, and stable puncture is achieved. The freezing heads are installed at the front end of the liquid storage container and used for installing the syringe needle, and a liquid conduction device is arranged at the end, provided with the syringe needle, of the corresponding freezing head. After the syringe needle is installed, the interior of the corresponding freezing head is in a conduction state, and low-temperature liquid in the liquid storage container flows into the syringe needle through the corresponding freezing head. When the syringe needle is not mounted, the interiors of the freezing heads are in a closed state. The freezing gun is suitable for cryotherapy or injection of large-area irregular-shaped lesion areas, and the number and position of freezing needles can be adjusted according to the shapes and sizes of the areas, so that cryotherapy is more precise and more efficient, and meanwhile the doctor-patient experience is effectively improved.