2 results about "Japonica rice" patented technology

The invention relates to a method of a molecular authenticating technique for the indica-japonica of old species of Yunnan Rice, which belongs to the breeding field in the technical field of agricultural biotechnology. The technical proposal is as follows: five grains of the material seeds to be authenticated are taken for germinating under the temperature of 20 to 35 DEG C; after the seeds geminate for five days, the leaves are taken for extracting the total DNA of the rice; the concentration of the DNA is detected and the DNA is quantificationally diluted to 25ng/ul; a specific primer JI designed according to the sequence of the specific long-arm section of the 10th chromosome of the rice is used for carrying out PCR reaction; gel electrophoresis is carried out on the 2 percent agarose (containing EtBr0.1ug/ml); the PCR product is detected on a UV transilluminator; and the indica-japonica characteristics of the material are authenticated according to the electrophoresis result. The indica type rice has two PCR products with the molecular weights being respectively 280bp and 380bp; and the japonica rice has one PCR product with the molecular weight of 280bp, thus authenticating the indica-japonica characteristics of the old species of Yunnan Rice. The method has the effects of being quickly and effectively authenticating the indica-japonica characteristics of the old of Yunnan Rice.