The invention relates to an electronegative single-layer organic photoconductor drum and a preparation method thereof, and belongs to the technical field of organic photoconductor drums. The electronegative single-layer organic photoconductor drum has the advantages that TPD-FCN is used as an electron transmission material, S-100 is used as a cavity transmission material, and TPD-FCN and S-100 have the approximate absolute values of oxidation-reduction potentials, and equivalent absolute values of electron/cavity transfer rates, so that the problems of low sensitivity, light exposure and high residue are solved when the single-layer functional composite organic photoconductor drum is applied to an electronegative laser printer; by adjusting the contents of components in a coating liquid, the photoelectric property of the prepared electronegative single-layer organic photoconductor drum reaches or approaches to the level of the multi-layer functional separation type electronegative organic photoconductor drum; compared with the multi-layer functional separation type electronegative organic photoconductor drum, the preparation process is simple, the production efficiency is high, the production cost is low, and the like.