1 results about "Novel gene" patented technology

The invention discloses a novel gene cloning method independent of enzymes. The novel gene cloning method comprises the following steps of amplifying a target gene and a plasmid vector with a primer;performing heat treatment on target gene amplification products and plasmid vector amplification products with iodine; performing hybridization pairing on target gene DNA fragments and plasmid vectorDNA fragments after heat treatment, to form a recombinant plasmid with a notch, containing the target gene; and transforming escherichia coli with the recombinant plasmid with a notch, containing thetarget gene, and repairing the notch of the recombinant plasmid to form a complete recombinant plasmid containing the target gene. In the manner, the best sulfo modifying and single-strand end lengthcontrol strategy is adopted, the positive cloning rate of the target gene is high, the operation flux is high, and the method is suitable for simultaneous cloning of a plurality of target genes.