The present invention relates to a canned pearl-like tendon ball which is prepared from the following raw materials in parts by weight: tendons, edible salt, white sugar, monosodium glutamate, gelatin, rana japonica oil, walnut powder, ginsengs, fructus amomi, nutmeg, alpinia officinarum Hance, bay leaves, cloves, Chinese prickly ash, star anise, cassia barks and osmanthus fragrans. The present invention also relates to a manufacturing method of the canned pearl-like tendon ball, which comprises the following step: clear water soaking, salt water boiling, segment cutting and shaping, dehydrating and air-drying, nutrient solution brewing, and canning and sterilizing. The canned pearl-like tendon ball has the beneficial effects that the prepared canned pearl-like tendon ball is delicious and tasty, perfect in color, fragrance and taste, complete in shape, rich in nutrition, and convenient in consumption, can be eaten directly, and is convenient to carry and simple and practicable in production process.