The invention provides a method for cultivating cruciferae plants for observing the plasmodiophoromycetes infection. Root organizations of the initially-pathogenetic cruciferae plants are taken to prepare a plasmodiophoromycetes solution, cruciferae seeds are sowed in quartz sand, and the quartz sand and the seeds are soaked with an MS basic culture solution; under the conditions that the illumination intensity is 100 micro-mol photons/(m<2>.s), the illumination time ranges from 16 h to 18 h, the temperature ranges from 20 DEG C to 22 DEG C, and the humidity is higher than 75%, the seeds are cultivated until seed germination and are grown into plants with two main leaves. The plasmodiophoromycetes solution is applied to the roots of the plants to conduct the infection, and then root systems of the infected plants are observed through a biological microscope in a grading mode until the infection stage is finished. The method is easy and convenient to operate, distinct in observation result, small in occupied space and low in test cost, and has the advantages of being safe in operation, simple, high in repeatability, convenient to observe and distinct in result.