4 results about "Sperm" patented technology

The invention belongs to the technical field of marine organisms, and concretely relates to a method for the efficient ultralow temperature cryopreservation of turbot sperms. The method comprises the following steps: mixing the seminal fluid of turbots with an antifreeze liquid according to a volume ratio of 3:1-4:1, carrying out two-step cooling treatment, carrying out one-step balancing, adding to liquid nitrogen (having a temperature of -196DEG C), and preserving; and the antifreeze liquid is composed of a diluent, an antifreeze agent and an additive. High-quality frozen sperms obtained through the method for the efficient ultralow temperature cryopreservation of turbot sperms are of great significance of the artificial breeding, germplasm preservation, heredity diversity and sustainable culturing of the turbots.

The invention relates to an isolated culture method of an unfertilized ovary of lilium, belonging to the technical field of plant haploid culture. The method provided by the invention comprises the following steps: with a flower bud one day before bloom as a testing piece, sterilizing and taking the ovary; transversely cutting the testing piece to sheets; then, inoculating a BDS induction medium with the ovary sheets to induce embryogenesis so as to obtain the embryo rate with high frequency, wherein averagely each ovary contains 108-223 embryo ovules; and then, transferring the induced ovary sheets to the BDS regeneration medium to obtain a regeneration plant with other ploidy such as ploidy, diploid and aneuploid through the path of embryogenesis. The method provided by the invention has important meaning for accelerating lilium breeding, germplasm innovation and basic research of genetics.

The invention discloses a smelt artificial propagation method. From the early June, the fodder containing more fish meal protein is used for the feeding of female parent fishes. While from the middle of September, the female parent fishes are fed on the fodder containing spirulina powder and aluzyme. After the parent fishes enter the gonad mature period, the better mature female parent fishes are selected and cultivated after the estrualization, and the sexually mature male parent fishes are selected and cultivated after the estrualization. After the cultivated parent fishes reach more than 60 % gonad maturity, the water temperature of a decorporation pool is dropped continuously for 2 days with the decline of 2 degree each day. Afterwards the constant temperature is kept for 4 to 6 days. During the ovulatory period of parent fish, the ovums and the sperms are artificially collected every two days. The dry artificial insemination method is used to obtain a germ cell, and the germ cell is put in a hatchery pond for breeding. With the invention, the smelt sverage absolute fecundity and the fertility rate can be enhanced, and a great number of fry can be obtained in each breeding. The scale of artificial propagation is increased, and the labour intensity of the artificial propagation is reduced. Thus a foundation for the large scale artificial culture of smelt is established.

The invention belongs to the technical field of sperm detection, and particularly relates to a non-diagnostic quantitative detection method for active oxygen of a single sperm and application of the non-diagnostic quantitative detection method. According to the invention, a movable miniature exciting light probe is creatively used and positioned near a single sperm, and a complete detection process is constructed and comprises the steps of processing, positioning, noise reduction, measurement, baseline processing and the like. Finally, the fluorescence intensity emitted by the single sperm is accurately quantified, and the intracellular active oxygen value of the sperm can be accurately given. According to the present invention, the active oxygen detection of the single sperm level is initiated in the field, the field blank is filled, the number of the sperms is not limited, and the method can be suitable for almost all patients including severe oligospermia.