2 results about "Subspecies" patented technology

The invention discloses an establishment method for a type 2 diabetes mellitus animal model. The establishment method comprises the following steps: (1) preparing a lipopolysaccharide solution with aconcentration of 100ug/ml by virtue of normal saline; (2) selecting male rats which are 4-6 weeks old, feeding by virtue of a common feed, and simultaneously injecting the lipopolysaccharide solution;(3) weighing the male rats after 28 days; (4) after the weighing, fasting for 8 hours, separating serum, and measuring blood glucose and insulin; (5) evaluating the resistance of the insulin and glucose stimulated insulin secretion capacity; (6) respectively intraperitoneally injecting an STZ solution into the male rats in the 30th day and the 32th day; and (7) determining that the establishmentof the type 2 diabetes mellitus animal model is finished when the random blood sugar is more than 16.8mmol/L. According to the establishment method, lipopolysaccharide in Escherichia coli 0111:B4 subspecies is selected and is matched with the feeding of the common feed and the STZ injection so as to successfully establish the type 2 diabetes mellitus animal mode; according to the establishment method, the operation is easy, the model establishment cost is low, the model establishment rate is high, and the model establishment period is short.

The invention relates to a selective separation medium for caprine mycoplasmal pneumonia subspecies and a preparation method of the selective separation medium, and belongs to the technical field of veterinary biology. The selective separation medium is prepared from components as follows: lactoalbumin hydrolysate, yeast extract powder, glucose, an MEM medium, a PPLO (pleuropneumonia-like organism) gravy powder, sodium pyruvate, a 1% phenol red solution, specific growth inhibition serum and deionized water. The preparation method comprises steps as follows: the lactoalbumin hydrolysate, the yeast extract powder, glucose, the MEM medium, the PPLO gravy powder, sodium pyruvate and the 1% phenol red solution are weighed in proportion and dissolved in the deionized water. Before use, healthy horse serum and the specific growth inhibition serum are added. The selective separation medium for the caprine mycoplasmal pneumonia subspecies is used for clinically separating and cultivating the caprine mycoplasmal pneumonia subspecies, increases the growth speed of mycoplasma in a separation process and increases the separation rate of the caprine mycoplasmal pneumonia subspecies, and the separation rate is 80% or higher.