Functional nano-carrier with escape capability of lysosome and preparation method of same

[0056] Preparation of functional drug-loaded lipid nanocarriers

[0057] Weigh 4mg of paclitaxel, 1mg of decanyl arginine, 75mg of phospholipids, and 25mg of medium-chain fatty acid glycerides, dissolve in 3mL of methanol, evaporate under reduced pressure in a 37°C water bath to form a film, and add 2.3mg / mL of bovine serum albumin aqueous solution 4mL, hydrated for 15min, sonicated with an ice bath probe (200w×300 times), squeezed through a 0.22μm microporous filter membrane, and obtained a functional drug-loaded lipid nanocarrier with an average particle size of 80-100nm and an encapsulation efficiency of 98.6%. .

[0061] Preparation of functional drug-loaded liposomes

[0062] Weigh 5 mg of doxorubicin, 2 mg of lauryl arginine, and 100 mg of phospholipids, dissolve them in 3 mL of ethanol, slowly drop them into 5 mL of distilled water while ultrasonicating, evaporate the ethanol under reduced pressure, and ultrasonicate with an ice-bath probe (200w×300 times) , extruding through a 0.22 μm microporous membrane to prepare functional drug-loaded liposomes with an average particle size of 60-80 nm and an encapsulation efficiency of 97.9%.

[0064] Weigh 100 mg of phospholipid, 5 mg of lauryl arginine and dissolve in 6 mL of ether, weigh 4 mg of mitoxantrone and dissolve in 2 mL of distilled water, slowly add to the ether solution while ultrasonicating to form a water-in-oil emulsion, evaporate under reduced pressure to Invert the phase to become an oil-in-water emulsion, add 2mL of distilled water, continue to evaporate under reduced pressure to remove ether, and replenish water to 4mL, ultrasonically (200w×300 times) with an ice-bath probe, squeeze through a 0.22μm microporous filter membrane, and dextran gel The column removes free mitoxantrone to prepare liposomes with a particle size of 60-80nm and an encapsulation efficiency of 99.2%.