Method for detecting alkaline phosphatase in body fluid
A phosphatase and alkaline technology, applied to the detection of alkaline phosphatase in body fluids, based on the field of bioluminescence quantitative detection of alkaline phosphatase, can solve the problems of high price, high analysis cost, interference with enzyme and substrate reactions, etc., to achieve Improve sensitivity and accuracy, strong anti-interference ability, and high sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0048] Example 1
[0049] Using bioluminescence method to detect the content of alkaline phosphatase in serum
[0050] (1) Dilute normal human serum 10 times with pure water, and then add a series of different concentrations of alkaline phosphatase to the serum so that the final concentration is 7.5×10 -2 , 3.75×10 -2 , 7.5×10 -3 , 3.75×10 -3 , 7.5×10 -4 , 3.75×10 -4 , 7.5×10 -5 , 3.75×10 -5 , 7.5×10 -6 , 3.75×10 7 And 1.85×10 -7 .
[0051] (2) Take 1000μL of serum sample, add 100μL of adenosine triphosphate solution to it and resuspend it, react for 30min at 37℃; then add 50μL of bioluminescence reagent (luciferin and luciferase), and measure the bioluminescence value with a portable ATP detector .
[0052] Take the concentration of alkaline phosphatase as the abscissa and the change of bioluminescence intensity as the ordinate to construct a standard curve. The content of unknown alkaline phosphatase in the serum can be detected by the standard curve. figure 2 It can be seen that the
Example Embodiment
[0053] Example 2
[0054] Linear relationship between adenosine triphosphate concentration and bioluminescence value
[0055] Dilute the adenosine triphosphate solution with Tris-HCl to concentrations of 0, 0.01, 0.1, 0.5, 1, 10, 20, 50, and 100μM. Take 50μL each of adenosine triphosphate with different concentrations, and then add bioluminescence containing luciferase and luciferin Reagent 50μL, mix well and quickly use a portable ATP detector to determine the bioluminescence value. A standard curve was constructed based on the relationship between luminescence value and ATP concentration. The linear relationship between adenosine triphosphate concentration and bioluminescence value is as image 3 Shown from image 3 It can be seen that as the concentration of ATP increases, the bioluminescence value becomes larger, which has a good linear relationship.
Example Embodiment
[0056] Example 3
[0057] The relationship between the content of alkaline phosphatase and the change of bioluminescence value
[0058] Dilute alkaline phosphatase with water to a concentration of 0,1.5×10 -5 , 3×10 -5 , 1.5×10 -4 , 3×10 -4 And 1.5×10 -3 U / mL, then take 100μL of alkaline phosphatase diluent, then add 50μL of 50μM ATP solution and mix well, react for 45min in a 37°C incubator, and finally add 50μL of bioluminescence reagent to mix well, pass the portable ATP detector Detect the bioluminescence value. From Figure 4 It can be seen that this method can detect 1.5×10 -5 U / mL alkaline phosphatase.
PUM
Property | Measurement | Unit |
---|---|---|
Particle size | aaaaa | aaaaa |
Specific saturation magnetization | aaaaa | aaaaa |
Concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap