Method for determining content of diminazene and antipyrine in diminazene particle

A technology of antipyrine and triazemidine, which is applied in the field of analytical chemistry, can solve the problems of no recorded detection method, poor preventive effect, etc., and achieves the effects of improving sensitivity and accuracy and simple operation.

Active Publication Date: 2012-08-15
HEBEI KEXING PHARMA
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Triazamidine belongs to the class of aromatic diamidines and is a broad-spectrum anti-blood protozoal drug traditionally used. It has therapeutic effects on Piroplasma, Trypanosoma and Anaplasma in livestock, but the preventive effect is poor.
Triazamidine granules with therapeutic effect, in which a certain amount of antipyrine is ad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining content of diminazene and antipyrine in diminazene particle
  • Method for determining content of diminazene and antipyrine in diminazene particle
  • Method for determining content of diminazene and antipyrine in diminazene particle

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example A method for determining the content of triazamidine and antipyrine in triazamidine granules

[0032] This method belongs to the HPLC method, and its chromatographic conditions are:

[0033] (1) Use octadecylsilane bonded silica gel as the filler Diamonsil C 18 Column (250mm×4.6mm, 5μm);

[0034] (2) The mobile phase volume is phosphate buffered saline solution: methanol = 27:10. Phosphate buffer is potassium dihydrogen phosphate solution (can also be replaced by sodium dihydrogen phosphate solution), specifically a 1.15% potassium dihydrogen phosphate aqueous solution, with 85% phosphoric acid to adjust the pH to 3.0;

[0035] The flow rate of the mobile phase used is 0.5mL / min, the injection volume is 20μL, and the column temperature is 30°C;

[0036] (3) The detection wavelength of the UV detector is 250nm.

[0037] 1. The establishment process of the method of the present invention:

[0038] 1. Instruments and reagents

[0039] Instrument: Shimadzu LC-2010A high perfor

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Wavelengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for determining content of diminazene and antipyrine in diminazene particles, and belongs to HPLC (high performance liquid chromatography). The chromatographic conditions of the method is as follows: (1) octadecyl silane-bonded silica gel is DiamonsilC18 column (250mm*4.6mm, 5 mum) of a filler; (2) the mobile phase volume ratio of phosphate buffered saline to carbinol is 27 to 10; and (3) the measurement wavelength of an ultraviolet detector is 250nm, the flow velocity of the mobile phase is 0.5mL/min, the introduced sample volume is 20 muL, and the column temperature is 30 DEG C. The detection method disclosed by the invention is simple in operation, and enhance the sensitivity and the accuracy of the detection. The method disclosed by the invention is suitable for determining the content of diminazene and antipyrine in diminazene particles.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner HEBEI KEXING PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap