Processing method of adipose tissue, mesenchymal stem cell, single processed adipose cell, and extracellular matrix

[0079] Example 1

[0080] 2.4 g of adipose tissue was washed repeatedly with physiological saline to remove tumescent fluid and leukocytes in the adipose tissue. Then, the washed adipose tissue is fully minced with scissors until it is in the form of meat paste, and the particle size of the adipose tissue is between 1-2 mm, and placed in a sterile container. Add 300 units of collagenase for injection (certificate of Chinese medicine: H31022658, the same below), after shaking and digesting at 37°C for 30 min, add 5 mL of human albumin with a mass fraction of 20% (certified of Chinese medicine: S10980016, the same below) for neutralization injection With collagenase, the total number of cells was about 2.35 × 10 after filtration through a 70 μm cell sieve 6 indivual.

[0081] Example 2

[0082] 2.4 g of adipose tissue was washed repeatedly with physiological saline to remove tumescent fluid and leukocytes in the adipose tissue. Then, the washed adipose tissue is fully minced with scissors until it is in the form of meat paste, and the particle size of the adipose tissue is between 1-2 mm, and placed in a sterile container. Add 300 units of collagenase for injection (H31022658) and 125,000 units of urokinase (H44020645), shake and digest at 37°C for 30 minutes, then add 10 mL of human serum albumin with a mass fraction of 20% for neutralization Collagenase and urokinase for injection, filtered through a 70μm cell sieve, the total number of cells was about 2.39×10 6 indivual.

[0083] Example 3

[0084]2.4 g of adipose tissue was repeatedly washed with physiological saline to remove tumescent fluid and leukocytes in the adipose tissue. Then, use scissors to fully cut into pieces, and after the washed adipose tissue is in the shape of meat paste, and the particle size of the adipose tissue is between 1-1.5 mm, it is placed in a sterile container. 300 units of collagenase for injection were added, and after 30 min of shaking at 37°C, 5 mL of human serum albumin with a mass fraction of 20% was added to neutralize the collagenase for injection. After centrifugation at 2400 r / min for 5 min, the cell pellet was collected. Add physiological saline to resuspend the cell pellet and filter it with a 70 μm pore size cell sieve to obtain a single cell suspension. Then centrifuge at 1200r / min to collect the precipitate, remove the supernatant, remove the collagenase for injection and human albumin, and obtain a total number of cells of about 2.16×10 6 indivua