Bacillus cereus NJSZ-13 and application thereof

A technology of Bacillus cereus and NJSZ-13, applied in the field of microorganisms, can solve the problems such as the lack of control of pine wood nematodes, and achieve the effects of good practicability, strong nematicidal activity, and good competitiveness.

Active Publication Date: 2017-03-22
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented Bacillus spore strain (BSC) isolate was found that it had greater resistance than other existing germs used for control on plants such as Pinus radiata or citrus trees. Its superiority over these new sources led researchers towards developing more effective ways to fight plant diseases caused by insect-borne parasites called phytopathogenic fungi.

Problems solved by technology

This patents discuss how controlling this type of insect called Bissosporium amplexum plays crucial role during the spread of diseases like phytophthora citriberis or Pierce's Disease. Biologically controlled substances such as necroticonworm mothers may cause harm if they enter into these areas where plants cannot survive without them. To address this problem, new methods involving natural enemy agents were developed based upon genetic engineering techniques.

Method used

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  • Bacillus cereus NJSZ-13 and application thereof
  • Bacillus cereus NJSZ-13 and application thereof
  • Bacillus cereus NJSZ-13 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Cultivation of test nematodes and preparation of nematode suspension

[0019] The pine wood nematodes tested were provided by Jiangsu Key Laboratory of Pest Invasion Prevention and Control, Nanjing Forestry University, and were isolated from black pine trees in Lianyungang, Jiangsu Province. The pine wood nematode was cultured with Botrytis cinerea (Botrytis cinerca). The nematodes were inoculated on a PDA plate covered with Botrytis cinerea, and placed in an incubator at 25°C for cultivation until the hyphae of Botrytis cinerea disappeared and the nematodes covered the plate. The nematodes were separated and collected by the Baermann funnel method, and then centrifuged, sterilized and concentrated to prepare a nematode suspension of about 20 nematodes / μL.

[0020] 2) Preparation of sterile fermentation filtrate and cell suspension of NJSZ-13

[0021] Bacillus cereus NJSZ-13 stored on beef extract peptone (NA) medium was placed in a 50 mL Erlenmeyer flask containing

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Abstract

The invention discloses pine wood nematode-killing Bacillus cereus, which is classified and named as Bacillus cereus NJSZ-13, and preserved in China type culture collection center, the preservation number is CCTCCM2016660, the preservation date is November 20, 2016, and the preservation address is Wuhan University in Wuhan in China. The Bacillus cereus NJSZ-13 isolated from bodies of pine trees has better competitive ability and colonization ability, and sterile fermentation broth and bacteria aqueous suspension of the Bacillus cereus NJSZ-13 both have strong nematicidal activity on pine wood nematodes. By experiments, 36h after the treatment, the killing rate of the sterile fermentation broth on the pine wood nematodes can reach 100%, bodies of the pine wood nematodes can be digested, 48h after the treatment, the killing rate of the bacteria aqueous suspension on the pine wood nematodes can reach 86.5%, and the pine wood nematode-killing Bacillus cereus has good practicability.

Description

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Claims

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Application Information

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Owner NANJING FORESTRY UNIV
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